Ship of the Line is the first expansion to Flying Colors. It primarily (although not exclusively) covers major fleet actions during the period of the American Revolution. The expansion includes sixteen total scenarios and adds over 100 new, individually named and rated ships as well at over 30 new commanders. Scenarios include:

1. Quiberon Bay, 20 November 1759
2. 1st Ushant, 27 July 1778
3. Rhode Island, 11 August 1778 (hypothetical)
4. Saint Lucia, 15 December 1778
5. Grenada, 6 July 1779
6.