By emitting high-pitched sounds and listening to the echoes, also known as sonar, microbats locate prey and other nearby objects. This is the process of echolocation, an ability they share with dolphins and whales. Two groups of moths exploit the bats' senses: tiger moths produce ultrasonic signals to warn the bats that the moths are chemically-protected (aposematism) (this was once thought to be the biological equivalent of "radar jamming", but this theory is still unconfirmed); the moths Noctuidae have a hearing organ called a tympanum which responds to an incoming bat signal by causing the moth's flight muscles to twitch erratically, sending the moth into random evasive manoeuvres.

Although the eyes of most microbat species are small and poorly developed, leading to poor visual acuity, it is incorrect to assume that they are nearly blind. Vision is used as an aid in navigation especially at long distances, beyond the range of echolocation. It has even been discovered that some species are able to detect ultraviolet light. Their senses of smell and hearing are excellent.